Origins of anti-DNA autoantibodies.

Origins of anti-DNA autoantibodies.

Intricacies of anti-DNA autoantibodies.

Repertoire cloning of lupus anti-DNA autoantibodies.

To investigate the autoantibody repertoire associated with SLE, we have created phage display IgG Fab libraries from two clinically active SLE patients and from the healthy identical twin of one of these patients. The libraries from the lupus discordant twins were found to both include unusually large representations of the V(H)5 gene family. By panning with DNA, the SLE libraries each yielded ...

The evolution of human anti-double-stranded DNA autoantibodies.

It has been proposed that the anti-double-stranded DNA (dsDNA) response in patients with systemic lupus erythematosus (SLE) is antigen driven and that DNA or nucleosomes select anti-DNA reactive, somatically mutated B cells. We have used site-directed mutagenesis to systematically revert the somatic mutations of two human anti-dsDNA antibodies from SLE patients to analyze the resulting changes ...

Origins and Evaluation of DNA Damage in Infertile Individual

Assisted reproductive techniques (ART) are considered as the first line of treatment in overcoming infertility. The scope of success of these methods depends on several factors including integrity of sperm and oocyte. Obviously, paternal genomic content has an important effect on fertilization potential in infertile couples. Thus, sperm DNA damage is a genomic disorder that exists in different ...